BCH 361 Lecture Notes - Lecture 6: Hydrolysis, Alpha Helix, Dithiothreitol
5 Feb 2017
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Primary structure- amino acid sequence--> secondary- alpha helix or beta pleated sheets --> tertiary structure- polypeptide chain--> quaternary structure- assembled subunits. The primary structure determines the structure and function of that protein. Single amino-acid changes in the primary structure (due to mutations) can lead to malfunction and diseases like sickle cell anemia and cystic fibrosis. 1)sequence can be determined by genes- each amino acid is encoded by a specific sequence of 3 nucleotides in dna. 2)the edman degredation procedure labels and removes only the amino terminal residure from a peptide, leaving other peptide bonds intact. Peptide is reacted with phenylisothiocyanate under milk alkaline conditions, converting the amino acid to a ptc adduct the peptide bond next to the ptc adduct is cleaved in acid. Enzymes called proteases catalyze the hydrolytic cleavage of peptide bonds. Bioinformatics: computationally compare the primary structure of any protein to:
