BCH 361 Lecture Notes - Lecture 14: Elisa, Western Blot, Cell Culture
5 Feb 2017
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Ligand 1 affects the binding affinity of a protein for another ligand 2. If ligand 1 = ligand 2, then this is homotropic regulation (e. g. o2 binding to hemoglobin) If ligand 1 is different from ligand 2, then heterotropic regulation (e. g. bpg binding to hemoglobin affects o2 binding) The effect may be positive or negative; i. e. , ligand 1 may induce a higher affinity for ligand 2 (positive) or lower affinity (negative) The hill equation produces a line plot, which reflect degree of interaction between n binding sites. The slope of the line is called nh. If positive cooperativity, then nh > 1 for a certain range of [l]. Nh < 1 for a certain range of [l]. There is extensive aggregation only when both alleles of the b subunit are of the mutant form. Thus, sickle-cell anemia is displayed in homozygous individuals: however, hemoglobin s in heterozygotes confer resistance to malaria.
