Class Notes (836,615)
Canada (509,866)
Biology (528)
BLG 311 (61)
Lecture

diabetes and genetics

2 Pages
58 Views
Unlock Document

Department
Biology
Course
BLG 311
Professor
Ross Mc Naughton
Semester
Fall

Description
Insulin and Genetic Engineering  Diabetes mellitus is the inability of beta cells of pancreas to produce insulin  Restriction enzymes/endonuclease cut DNA at specific recognition sites o This produces either "sticky ends" or "blunt ends" o DNA ligase can be used to re-join the ends  Recombinant DNA technology combines the DNA from two different organisms  Reverse transcriptase catalyses the formation of DNA from mRNA  Vector is a gene carrier. It will carry a human gene into the cell of a bacterium or yeast that will be used to make human protein. Produces no benefit for viruses / carrier  Plasmid, circular strand of DNA, are useful vectors to make human protein from bacteria  Transgenic organisms contain another species DNA Integration Link [EXAM] Remove a particular gene from the DNA of an animal cell  Locate with the use of a gene probe  Use restriction enzymes  Use endonucleases to cut at specific base sequence by hydrolysing  Breaking sugar-phosphate bonds [EXAM] Insert this gene into the genetic material of a bacterium  Same restriction enzymes  Cut at same base sequence in bacterial DNA  Leaving sticky ends/hydrogen bonds break  Join/splice with ligase  Use of plasmid Task to find and insert the gene into bacterium for Insulin production  Isolate human gene, e.g. insulin, by using cytoplasmic mRNA (no introns)  Reverse transcriptase, taken fr
More Less

Related notes for BLG 311

Log In


OR

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


OR

By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.


Submit