BME 639 Lecture Notes - Lecture 12: Bode Plot

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orangeminnow913

18 Oct 2017

School

Ryerson University

Department

Biomedical Engineering

Course

BME 639

Professor

Soosan Beheshti

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Document Summary

To have the error smaller than 10%, the gain has to be larger than 18. The gain range satisfying requirements (i. e. stable and error < 10%) is: 18 25. 2 when = 18, the system gain margin is equal to: Recall that gain in db is defined as 20: / , hence the gain margin is 2. 92 db. Note that the stable system will have a gain margin in v/v units as > 1, and in db units as > 0 db. To have the error equal to 5% the gain would have to be : To have the error equal to or smaller than 5%, the gain has to be equal to or larger than 38, but that would be outside the stable range. Note that the unstable system will have a gain margin in v/v units as < 1, i. e. fractional, and in db units as < 0 db, i. e.

Related Questions

please answer all the question answering only one question is not helpful .
Practice Calculations on Serial Dilutionsâbe sure to include the amount of water added and include all units

17. Calculate and describe (or diagram) how you would use a 10% SDS solution to prepare a final volume of 20 ml of each of the following solutions: 8%, 4%, 2%, 1% 0.5%.

18. Calculate and describe (or diagram) how you would use a 5 M Tris solution to prepare 200 ml of each of the following solutions: 1M, 100 mM ,10 mM , 1 mM, 0.1 mM.

How much of the first dilution should you make to perform the following dilutions?

19. 300 ml of a 1:5 dilution

20. 100 ml of a 1:10 dilution

21. What is the minimum volume you should pipette with the following? (include units with your answers):

P2 P10 P20 P100 P200 P1000

22. How accurate was your pipetting?

23. Was it better with the larger volume or smaller volume pipettor? Provide rationale.

(If there was a consistent problem with one pipettor please tell the instructor.)

Standard Curves

24. Complete a standard curve for the data above and tape a printed version into your notebook.

25. What is the R-squared value of your trend line? Is this good or badâ¦ i.e. would you repeat the assay?

26. What is the protein concentration of (Explain/show your calculations.)

Unknown #1 = Unknown #2 =

Data for DNA Concentration Procedure

Attach a labeled printout of the plate reader raw data as well as your generated standard curve to this lab.

26. What is the concentration of your unknown DNA sample?

27. What is the R ² for your standard curve data? Why is it important that the R² is >0.95?

28. Comment on the purity of your DNA... discuss- How can you determine the purity of DNA? What are common contaminants of DNA purified from cells?

Case History- An 11-month old Boy who's blue and short of breath

Chief Complaint: An 11-month old boy brought to the pediatric clinic with difficulty breathing.

History: Austin Green, an 11-month old Caucasian boy was brought by his foster parents to the Pediatric clinic with comnplaint of dyspnea (i.e difficulty breathing) and fatigue. These episodes last for several minutes, occur daily, and happen most copmmonly during exertion (i.e exercise). Austin spent the first 9 months of his life with hisbiological parents, but recent social problems have necessitated that he receive foster care.His foster parents have had him for the past month, and they have note that these "breathing spells" have become more frequent and severe over period of time. They also note that Austinb's shortness of breath diminishes somewhat if he lies down. Interestingly, he frequently assumes a squatting position during these episodes, and this seems to reduce hsi symptoms as well. His foster parents report that Austin has had no nausea, vomiting, change in appetie, or change in bowel or bladder habits. He is currently on no medications and no known allergies. There is no medical information available about any othefr member of Austin's biological family.

Lab. test results:

Hematocrit 43% normal (37-47%)

Hemoglobin 14mg/dl (normal 12-16ml/dl)

PH =7.21 (normal range=7.35 to 7.45)

[HCO^-3]=18meq/L (normal range=22 to 26 me=Eq/L)

1. The cardiologist ordered X-ray that showed decreased blood flow to the lungs. A follow-up echocardiogram revealed the following abnormalities in Austin's heart. (A) a narrowing of pulmonic valve, (B) a thickening of right ventriclewall, (C) a ventricular septal hole allowing direct exchange of blood between the right and the left ventricles, (D) an aorta that was shifted in position so that it was over-riding the right venbtricl, allowing it to recieve blood from both the left and the right ventricle.

What is the name of Austin's condition?

2. What is specifically causing the heart murmur?

3. Why did the chest X-ray show freduced pulmonary blood flow?

4. Austin has a thickening right ventriclewall. what type of hypertropy is this?

Why does Austin have this particular type of ventricular hypertrophy? BE SPECIFIC

5. Why di Austin's EGG show a right-shufted ventricular depolarization axis?

6. Which way will blood typically flow through Austin's ventricular septal defect(i.e from the left ventricle to the right ventricle, or vice versa? explain your answer in detail

7. Why is Austin cyanotic? BE SPECIFIC

8. Why does supplemental oxygen therapy did not correct Austin's cyanosis? BE SPECIFIC

9. How might the squatting position help relieve Auatin's dyspnea?

10. Why is Austin's...

(a) arterial pCO2 higher than normal?

(b). arterial [HCO^-3] lower than normal?

(c). arterial PH lower than normal?

11. Describe treatments for Austin's condition?

Please answer all the questions answering only one question is not helpful :

Calculations on Multiple Stock Solutionsâbe sure to include the amount of water added and include all units

11. Calculate how to use the 1 M Tris and 0.2 M EDTA stock solutions to make the 400 ml of a of 10 mM Tris and 1 mM EDTA solution.

12. Calculate how to use a 3 M NaCl and 10% NP40 stock solutions to make a 500 ml of a 300 mM NaCl and a 0.2% NP40 working solution.

Calculations on Simple Dilutionsâbe sure to include the amount of water added and include all units

13. Calculate the volumes necessary to make 200 ml of a 1:5 dilution of a sample.

14. Calculate the volumes necessary to make 50 ml of a 1:8 dilution of a sample

15. Calculate how you would dilute a cell suspension containing 3,000,000 cells/ml to obtain 10 ml with 100,000 cells/ml

16. Calculate the volume of a cell suspension containing 800,000 cells/ml you would need to obtain a total of 250,000 cells

Practice Calculations on Serial Dilutionsâbe sure to include the amount of water added and include all units