Chemical Laboratory Technician BIC472 Lecture Notes - Alkaline Phosphatase, Reaction Rate


Department
Chemical Laboratory Technician
Course Code
Chemical Laboratory Technician BIC472
Professor
c A R L O B A S T I N U T T O

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BIC472
Students name: Daniel Katsafados Partner: Josh G
Assignment 6 – ENZYMATIC PROPERTIES
Total Marks: 14
You are required to plot your data to determine some of the properties of calf-
intestinal phosphatase. Include tables of your raw data and calculated values, and
show one sample of each calculation performed. The plots required and the
information to be obtained are summarized below:
PART A (4 marks)
Tube Temp. Abs at
410 nm
nmoles of
pNP
Velocity
(nmol/min)
10ºC 0.25 88.30 5.90
222ºC 0.35 123.6 8.40
335ºC 0.52 183.7 12.2
450ºC 0.47 166.0 11.1
560ºC 0.23 81.30 5.42
637ºC 0.00 0 0
1. Calculate the reaction velocity (nmol/min.) in each tube.
**Sample Calculation:
Tube 1:
A=2-log%T
A=2-log 56.5
A=0.2480
A= 0.25
[P] = A/18400M-1 X 1.0cm
[P] = 0.25/18400M-1cm-1 X 1.0cm
[P] = 0.000013587mol
nmol P= 0.000013587mol X 10 9nmol/mol X 0.0065L
nmol p= 88.3
Velocity = nmol [P]/ time
Velocity = 88.3nmol/15min
Velocity = 5.9nmol/min
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