BPK 143 Lecture Notes - Lecture 2: Sciatic Nerve, Supinator Muscle, Sensory Cortex
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An Hfr strain of genotype j+ k+ l+ m- strs is mated with an F- strain that is j- k- l- m+ strr. At a number of times after they are mixed, the culture is agitated to separate mating cells, which are then plated out on agar media of three types, where nutrient J allows the growth of j-cells, nutrient K of k-cells, nutrient L of l- cells, and nutrient M of m-cells. In the table below, â+â indicates the presence of streptomycin or the indicated nutrient, and â-â indicates its absence.
Medium | Str | J | K | L | M |
1 | + | + | + | - | + |
2 | + | - | + | + | + |
3 | + | + | - | + | + |
a. What donor genes are being selected on each type of agar?
b. The following table shows the number of colonies on each type of agar for samples taken at the indicated times after the initiation of mating. Use this information to determine the order of genes j, k, and l.
Sampling Time (min) | NUMBER OF COLONIES | ||
Medium 1 | Medium 2 | Medium 3 | |
0 | 0 | 0 | 0 |
5 | 0 | 0 | 0 |
7.5 | 100 | 0 | 0 |
10 | 200 | 0 | 0 |
12.5 | 300 | 0 | 75 |
15 | 400 | 0 | 150 |
17.5 | 400 | 50 | 225 |
20 | 400 | 100 | 250 |
25 | 400 | 100 | 250 |
c. From each of the 25 minute plates, 100 colonies are picked and transferred to a dish containing agar with all the nutrients except M. The number of colonies that grew on these plates is 89 of the 100 colonies from Medium 1, 51 of the 100 colonies from medium 2, and 8 of the 100 colonies from the Medium 3 plate. Using this data, fit gene m into the sequence of j, k, and l. Briefly explain your reasoning.
Shock Case Study (15 pts)
HPI. Mrs. K is a 22 y/o college student, rushed to the ED 35 minutes after sustaining multiple stab wounds to the chest and abdomen by an unidentified assailant. A witness called 911. Paramedics arriving at the scene found the victim to be in severe acute distress.
Vital signs were as follows: HR 128 (baseline 80), BP 80/55 (baseline 115/80), RR 37 and labored. Chest auscultation revealed decreased breath sounds in the R lung consistent with basilar atelectasis (ie. collapsed R lung). Pupils were equal, round, reactive to light, and accommodation. Her LOC was reported as âawake, slightly confused, and complaining of severe chest and abdominal pain.â Pedal pulses were absent, radial pulses were weak, and carotid pulses were palpable. The patient was immediately started on IV Lactated Ringerâs solution at a rate of 150 mL/hr.
An ECG monitor placed at the scene of the attack revealed that the patient has developed sinus tachycardia. She was tachypneic, became short of breath with conversation and reported her heart was pounding out of her chest. She appeared to be very anxious and continued to c/o pain. Her skin was cool and nail beds were pale but not cyanotic. Skin turgor was poor. Peripheral pulses were absent with the exception of a thread, brachial pulse. Capillary refill time was 7-8 seconds. Doppler ultrasound had been required to obtain an accurate BP reading. The patientâs skin was cool and clammy. There was a significant amount of blood on her dress and on the pavement where she was lying.
Question 2. What is the pathophysiologic sequence of events for shock? (2pts)
Question 3. What type of shock does this patient seem to have? What is your rationale? (2 pts)
Question 4. Does this patient need a blood transfusion? Provide rationale for your answer. (2pts)
During transport to the hospital, vital signs were reassessed: HR 138, BP 75/50, RR 38 with confusion. Patient was diagnosed with hypovolemic shock and IV fluids were doubled. Oxygen was started at 3L/min by nasal cannula. ER physicians chose not to start a central venous line. An indwelling foley catheter was inserted with return of 180mL of amber colored urine. Urine output measured over the next hour was 14mL. Patient was taken to the OR for surgical correction of lacerations to the right lung, liver and pancreas. In total, patient received 1L of Lactated Ringers.
Table 1.
Class | ||||
---|---|---|---|---|
Parameter | I | II | III | IV |
Blood loss (ml) | <750 | 750â1500 | 1500â2000 | >2000 |
Blood loss (%) | <15% | 15â30% | 30â40% | >40% |
Pulse rate (beats/min) | <100 | >100 | >120 | >140 |
Blood pressure | Normal | Decreased | Decreased | Decreased |
Respiratory rate (breaths/min) | 14â20 | 20â30 | 30â40 | >35 |
Urine output (ml/hour) | >30 | 20â30 | 5â15 | Negligible |
CNS symptoms | Normal | Anxious | Confused | Lethargic |