MBB 222 Lecture Notes - Lecture 17: Rna Splicing, Dna Supercoil, Pentose

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Dna is the genetic material of all free-living organisms (some viruses which are not necessarily (cid:862)li(cid:448)i(cid:374)g o(cid:396)ga(cid:374)is(cid:373)s,(cid:863) ha(cid:448)e rna ge(cid:374)o(cid:373)es(cid:895) The part of a dna molecule that encodes the information required for producing a functional product (> rna > protein) is called a gene. Primary structure: the nucleotide sequence, e. g. : 5"-gcggcaatcgta-(cid:1007)". Secondary structure: any regular, stable structure adopted by a segment of dna, typically a base-paired double helix. Tertiary structure: the 3d fold - the complex folding of: dna into bacterial nucleoids (supercoiled) or eukaryotic chromatin, or rna into trna and other molecules. Quaternary structure ribosomes, spliceosomes, risc (rna-induced silencing complex) The basic building block of dna is a nucleotide, which is comprised of: phosphate + sugar + base. The pentose sugar is ribose in rna and deo(cid:454)(cid:455)ribose in dna; has a 5" and 3" end. Carbons in the ribose or deo(cid:454)(cid:455)(cid:396)i(cid:271)ose a(cid:396)e (cid:374)u(cid:373)(cid:271)e(cid:396)ed (cid:449)ith p(cid:396)i(cid:373)es (cid:894)"(cid:895) to disti(cid:374)guish the(cid:373) f(cid:396)o(cid:373) atoms in the bases.

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