MBB 222 Lecture Notes - Lecture 8: Ramachandran Plot, Conjugate Acid, Periplasm

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Disulfide bonds are important for protein stability the cytoplasm is a reducing environment disulfide bonds cannot form and therefore proteins requiring disulfide bonds are not stable in the cytoplasm. Disulfide bonds only form in an oxidizing environment such as the lumen of the rough endoplasmic reticulum of eukaryotic cells or the periplasm of bacteria, or secreted by bacteria into the extracellular space. Disulfide bonds form after the protein has reached its folded form they stabilize the folded form. Hydrophobic amino acids are insoluble in water and tend to cluster on the inside of proteins van der waals interactions. All free amino acids (i. e. , not within a peptide) have a minimum of two ionizable groups: the backbone a-amino and a-carboxyl groups that are attached to ca. Free amino acids lacking an ionizable side chain (e. g. , val) exists as the zwitterion. Some polar side chains are also ionizable (e. g. , tyr, cys) but they are not ionized at neutral ph.

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