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Class Notes for Molec Biol & Biochem at Simon Fraser University

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MBB 222 Lecture 17: Lectures 17-18 Part 4_ Protein Techniques
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Lectures 17-18 Part 4: Protein Techniques - NMR spectroscopy, in contrast, uses a high-strength magnetic field to gather information on the relative positions of certain atoms in a protein solution. - In cases where it has...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 17: Column Chromatography, Protein Purification, Differential Centrifugation
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Lectures 17-18 Part 1: Protein Techniques Learning outcomes - Understand in general terms the various steps in protein purification, from lysing cells, fractionating (differential centrifugation), isolatingpurifying protei...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 33: Signal Peptide, Nuclear Pore, Prokaryote
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Lecture 33: Signal Peptides - There are large differences between the eukaryotic and prokaryotic ribosome - This makes protein synthesis a good target for drugs - Many antibiotics target the bacterial ribosome or translati...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 17: Two-Dimensional Gel Electrophoresis, Ionization, Mass Spectrometry
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Lectures 17-18 Part 3: Protein Techniques - Distinguish between an analytical technique (e.g., A 280, SDS-PAGE, 2D gel electrophoresis, immuneWestern blot, mass spectrometry) and a preparative one (centrifugation, salting ...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 17: X-Ray Crystallography, Cholera Toxin, Antigen
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Lectures 17-18 Part 5: Protein Techniques A model of the protein is built by fitting the polypeptide chain into the regions of electron density. In this example, only a small portion of the protein is shown. - expose prote...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 17: Polyacrylamide Gel Electrophoresis, Two-Dimensional Gel Electrophoresis, Affinity Chromatography
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Lectures 17-18 Part 2: Protein Techniques Gel filtration chromatography (aka size exclusion): - separates proteins based on size. Large proteins cannot enter the pores of the gel matrix beads and elute from the column firs...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 32: World Federation Of Trade Unions, Transcription Preinitiation Complex, Eif2
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Lecture 32 Part 2: Ribosomes - Nothing like the Shine-Dalgarno sequence. - eIF4A is a helicase to unwind secondary structures in the mRNA. - Proteins (initiation factors) and the initiation Met-tRNA team up with the small ...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 31: Rna Polymerase Iii, Rna Polymerase Ii, Rna Polymerase I
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Lecture 31 Part 1: Translation Eukaryotes have three different RNA polymerases (RNAPs) for synthesis of different RNAs - RNA polymerase I (RNAP I) in nucleolus synthesizes rRNA part of ribosomes - RNA pol II (RNAP II) in n...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 32: Peptidyl Transferase, Stop Codon, World Federation Of Trade Unions
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Lecture 32 Part 3: Ribosomes This shift requires a translocase, EF-G, and energy from GTP hydrolysis. EF-G mimics the structure of the EF-Tu- tRNA complex, which may allow it to bind to the A-site and displace the peptidyl...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 31: Inosine, Transfer Rna, Endonuclease
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Lecture 31 Part 2: Translation tRNAs loaded with amino acids translate mRNA codons The tRNA uses base-pairing to recognize the codon in the mRNA, linking RNA sequence to amino acid sequence Aminoacyl-tRNA synthetases load ...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 30: Guanosine Monophosphate, Phosphodiester Bond, Spliceosome
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Lecture 30 Part 4: Splicing Proposed mechanism for bringing the 5 and 3 splice sites together - splicing occurs during transcription - must bring the 5 and 3 splice sites together to ligate the upstream and downstream exon...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 29: Leucine Zipper, Coiled Coil, Regulatory Sequence
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Lecture 29 Part 5: Eukaryotic Transcription Common DNA binding motifs: the leucine zipper - the leucine zipper is a coiled coil formed by two -helices that dimerize through hydrophobic interactions between leucine side cha...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 30: Phosphodiester Bond, Spliceosome, Transesterification
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Lecture 30 Part 3: Splicing Splice sites in spliceosome-mediated introns are conserved in eukaryotes AGGU and AGG sequences mark where splicing will occur (at the down arrows). Splicing must be very precise. E1 is exon 1, ...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 30: Phillip Allen Sharp, Nucleotidyltransferase, Ribonuclease P
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Lecture 30 Part 2: Splicing Not just mRNA that get spliced, eg tRNA Maturation 1. RNAse P is a ribozyme 2. tRNA nucleotidyltransferase adds CCA to 3 end with no template 3. Splicing in this case takes place outside the nuc...

Molec Biol & Biochem
MBB 222
Adam Barlev
MBB 222 Lecture Notes - Lecture 28: Lac Operon, Lac Repressor, Camp Receptor Protein
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find more resources at oneclass.com Lecture 28 Part 4: Lac Operon The two-fold symmetry axis suggested the repressor binds with two-fold symmetry. The lac repressor binds DNA via a ...

Molec Biol & Biochem
MBB 222
Adam Barlev
Permachart - Marketing Reference Guide: Extensor Carpi Ulnaris Muscle, Flexor Pollicis Longus Muscle, Abductor Digiti Minimi Muscle Of Hand

perm ach artsMT T rigger Points II: Extremities T rigger Points II: Extremities ...

ANAT - Anatomy
ANAT 14
All Professors
ANAT - Anatomy
ANAT 14
All Professors
ANAT - Anatomy
ANAT 14
All Professors
Permachart - Marketing Reference Guide: Sacral Plexus, Lumbar Plexus, Sympathetic Ganglion

pe rm a ch ar ts MT Spinal Cord 2nd EDITION Figure ...

ANAT - Anatomy
ANAT 14
All Professors
Multicellular Biology - Reference Guides

This Guide is the progressive companion to the Cellular Biology title. The complexities of human physiology are made much clearer through the bright graphics and intelligently coordinated text boxes and glossaries that are...

ANAT - Anatomy
ANAT 14
All Professors
Permachart - Marketing Reference Guide: Levator Veli Palatini, Orbicularis Oculi Muscle, Scalene Muscles

Muscular System: pe rm ac ha rts MT Head & Torso 2nd EDITION ...

ANAT - Anatomy
ANAT 14
All Professors
ANAT - Anatomy
ANAT 14
All Professors
ANAT - Anatomy
ANAT 14
All Professors
Permachart - Marketing Reference Guide: Heart Valve, Inferior Vena Cava, Pulmonary Valve

permacharts TM Cardiovascular Physiology 2nd EDITION HEART STRUCTURE & FUNCTION ...

ANAT - Anatomy
ANAT 14
All Professors
ANAT - Anatomy
ANAT 14
All Professors
Permachart - Marketing Reference Guide: Meiosis, Nuclear Membrane, Nuclear Pore

l e a r n • r e f e r e n c e • r e v i e w permacharts TM CCellular Biology BIOLOGY BASICS C...

ANAT - Anatomy
ANAT 14
All Professors
Cellular Respiration - Reference Guides

The specific aspects of cellular respiration are explained in this helpful Guide. The fundamental processes such as the electron transport chain, proton pumps, ATP synthetase, and glycolysis are each described with helpful...

ANAT - Anatomy
ANAT 14
All Professors
Permachart - Marketing Reference Guide: Submandibular Duct, Parotid Duct, Palatine Tonsil

pe rm a ch a rtsMT DDigestive System 2nd EDITION Fi...

ANAT - Anatomy
ANAT 14
All Professors
Permachart - Marketing Reference Guide: Antigen-Presenting Cell, Memory T Cell, Clonal Deletion

permacharts TM IImmunology 2nd EDITION G ENERAL INFORMATION - AN OVERVIEW ...

ANAT - Anatomy
ANAT 14
All Professors
Permachart - Marketing Reference Guide: Superior Frontal Sulcus, Inferior Parietal Lobule, Superior Frontal Gyrus

p erm ac hartsMT Brain 2nd EDITION FIGURE 1: GROSS ANATOMY (LATERAL VIEW) ...

ANAT - Anatomy
ANAT 173
All Professors
Permachart - Marketing Reference Guide: Superficial Palmar Arch, Costocervical Trunk, Thyrocervical Trunk

p erm a ch ar tsMT CCirculatory System 2nd EDITION Figure...

Biology
BI 361
All Professors

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