CHEM 3400 Lecture Notes - Lecture 3: Theoretical Plate, Miscibility, Elution

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Some techniques will not be chromatography as there is no staionary phase (ce,fff). Basic components of chromatography - sample, column, detector. Chrom is analyical (small, good) - qualitaively it can idenify unknown compounds (requires detector that yields structural informaion), quanitaively it can quanify known compounds (requires selecive detector and standards). Chrom is preparaive (bigger, worse)- mostly used in industrial producion, chemical synthesis, sample preparaion. You can eliminate components that can harm experiment. Analyical separaiond - you can analyze muliple components at once. Chromaic retenion - mobile phase and staionary phase. Mobile phase - goes through the column; transport the analytes through the column (eluion) Staionary phase - doesn"t move, stays where it is; holds back the analytes (retenion) Substances start interacing with staionary phase (where separaion begins to take place), one compound moves through faster and another moves through slower (separaion) Each analyte behaves diferently regarding the balance between eluion and retenion -