BIOL 2000 Lecture Notes - Antisense Rna, Rna Interference, Gene Silencing

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Published on 12 Apr 2013
School
Department
Course
Chapter 8-4
Small RNAs were the breakthrough of the year
Non-coding RNAs
Small RNAS control gene expression at all levels
DNA
o Structure of chromatin
RNA
o Many different types of RNAs affected
Protein synthesis
o Translation
Templates
Different strands serve as templates for different gens but only one strand of
RNA is normally produced
o No anti-sense RNA strand produced
o Double stranded RNA only produced by artificially
Trigger production of gene regulation
Trans-genetics
Introduce additional genes into a cell or
organism
o Want to enhance phenotype by
allowing more selection
Unusual insertion sites for introduced
genes may generate double-stranded RNA
(an “artificial situation”)
o Not much control of where it
integrates
o Transgene works from left to right
o Normal gene works from right to left
Should stop at end of gene- does not- keeps going through
transgene
Creates complementary strand of RNA
Creates a double stranded RNA
Trans-genetics in petunias
Intention of enhancing purple pigment
Shutting of purple pigment phenotypes in certain areas
o Suppression
Similar suppression of phenotypes can be induced by injecting cells with
double stranded RNA molecules
RNA interference (RNAi)
Gene silencing mediated through
small RNA molecules
Double stranded RNA acts as the
trigger mechanism
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Document Summary

Small rnas were the breakthrough of the year. Small rnas control gene expression at all levels. Rna: many different types of rnas affected. Different strands serve as templates for different gens but only one strand of. Rna is normally produced: no anti-sense rna strand produced, double stranded rna only produced by artificially. Introduce additional genes into a cell or organism: want to enhance phenotype by allowing more selection. Unusual insertion sites for introduced genes may generate double-stranded rna (an artificial situation : not much control of where it integrates, transgene works from left to right, normal gene works from right to left. Should stop at end of gene- does not- keeps going through transgene. Shutting of purple pigment phenotypes in certain areas: suppression. Similar suppression of phenotypes can be induced by injecting cells with double stranded rna molecules. Gene silencing mediated through small rna molecules. Double stranded rna acts as the trigger mechanism.

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