BIOL 2000 Lecture Notes - Petri Dish, Dna Replication, Escherichia Coli

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4 Mar 2013
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Chapter 5- genetics of bacteria and their viruses. How do bacteria transfer dna to other cells: bacteriophage. Attaches to the outside of cell, injects their dna into the cell: conjugation. Fruits of dna technology made possible by bacterial genetics: modern gene cloning techniques. Propagation of human dna fragments into e. coli. Dividing bacterial cells: rapid exponential growth, easy to replicate in small time frame, looks for rare mutations easily (as it is so fast to replicate) Properties of bacteria: single celled, prokaryotic (no nucleus or membrane bound organelles, have only 1 circular chromosome. Cell division will occur: resulting in two identical daughter cells. Identical to each other and parent provided there are no mutations. Colonial expansion- continual rounds of dna replication: do not undergo meiosis. Single cells are not visible to naked eye: all the colonies will have the same genotype, each different colony will have different genotypes as they are a new combination of inputs.

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