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Chapter 5-3.docx

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BIOL 2000
Joseph Rasmussen

Chapter 5-3 What if we study different Hfr strains?  Questions: Are the relative positions of genes, the position of the origin, and the direction of transfer the same in each Hfr strain? o Experiment: Consider 5 different Hfr strains derived from the same F+ strain and determine the order of the gene transfer from each strain into an F- strain o Strain 1:  A will always transfer  E did not transfer because it is too far around the allele o Strain 2:  Different cut site  Different insertion site  Continuous DNA o Proves that chromosomal DNA must be circular  Results o The fist gene to enter may differ o The relative position of each market gene is constant  B is always between C and A o The overall directionality of transfer can differ- compare strains 2 and 4  Directionality is opposite  Orientation can be flipped during integration  Arrowhead will determine origin, and it will point towards recipient cell Question: What controls the positions and insertion and orientation of F Plasmid?  There are multiple insertion sequences (IS) in the E.Coli genome  A SCO inserts F at a specific locus o Orientation of IS determines the order of chromosomal gene transfer but not F plasmid gene transfer  If there are multiple regions where they can integrated,
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