The overall 3D shape of the entire protein
o Globular proteins
Has alpha-helical regions coming together in 3D
Where does a small molecule bind?
Where are the precise positions of the atoms in 3D
o We will know exactly what it is made up of
Several proteins coming together
Intercations between multiple polypeptides
o For oxygen transport
o Four separate polypeptides for the complex
2 of each alpha and beta
o identical subunits
o different subunits (different polypeptides)
The genetic code
How id the sequence in DNA eventually converted into linear amino acid
sequence (primary structure of proteins?
o How is the mRNA template read?
1) How many nucleotides specify one amino acid in a protein?
Length of mRNA sequence that specifies one amino acid
Codon length possibilities
o 1 nt= 4 combinations (4 amino acids)
o 2 nt = 4x4 = 16 amino acids
o 3 nt = 4x4x4 = 64 amino acids (maximally)
only need 20 to specify
Codons are 3 nt long How are codons read?
o Overlapping genetic code
One nt affects amino
acid composition at
more then one
Each nt is only part of one codon
o Dependent on the reader