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BIOCH 200 (January 15, 2014) - Nucleotides and Nucleic Acids

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University of Alberta
Walter Dixon

BIOCH 200 Nucleotides and Nucleic Acids (con’t) • *The most important factor in stabilizing the double helix structure of DNA are the hydrophobic interactions that occur between the paired bases and water molecules. o In single stranded DNA, the bases interact with water and no helical structure is witnessed. • Why does ssDNA absorb more UV light than dsDNA? o *Since the bases of the single-stranded DNA are not linked to a complementary base, they are less constrained than bases in double- stranded DNA. Since ssDNA is less constrained it has more resonance capabilities for electron delocalization than dsDNA. • *(Correction for mistake in last set of notes) Generally, DNA (both kinds) have a wavelength absorption ratio between 260nm:280nm of 2:1. If there is any protein contamination, there would be more absorption of 280nm wavelengths of light and therefore the ratio of 2 would decrease accordingly. • Protein UV light absorption is due to side chains. Protein examples: o Tryptophan o Tyrosine • *The cytosine  uracil deamination reaction is an important reaction to remember! • *An important characteristic of pyrimidine: o They have 2 nitrogen atoms that are a part of the heterocyclic ring • *Thymine + ribose = ribothymidine o IT IS NOT thymidine. Thymidine is automatically assumed to the be = to thymine + deoxyribose Catabolic process – process of energy generation Anabolic process – process of
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