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Lecture

BIOCH 200 (January 22, 2014) - Nuceotides and Nucleic Acids

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Department
Biochemistry
Course
BIOCH200
Professor
Walter Dixon
Semester
Winter

Description
BIOCH 200 – Nucleotides and Nucleic Acids con’t (January 22, 2014) DNA Melting • *Tm= midpoint of melting (halfway between denaturing and annealing) o *Varies based on base composition  *The higher the GC content, the stronger the base stacking forces, the more stable the DNA structure, and therefore the higher the temperature is needed for denaturing (higher m ) • *Why? Because there are 3 HBonds that occur between guanine and cytosine and only 2 HBonds that occur between adenine and thymine • *GC has what is called “stronger base stacking interactions” • H-bonds and base-stacking are weak forces  this is why it is pretty easy to denature DNA • Levels of absorbance o *High?  hyperchromicity o *Low?  hypochromicity • Splitting of dsDNA = denaturing o *Required for DNA replication or transcription o When protein denatures, it denatures fastest at zones with a higher proportion of AT base pairs. • 2x ssDNA  dsDNA = annealing o *DNA annealing is slow at first but once the initial base pairings are made, annealing happens at an accelerated rate  (L
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