BIOIN301 Lecture Notes - Lecture 1: Start Codon, National Center For Biotechnology Information, Intron
Document Summary
O simple gene structure, small genome (~10millionbp), no introns, genes are called orf, high coding density, genes overlap, genes are short. Scan forward strand for start codon, then find the stop codon. Tata__orf: orf finding (manually) reverse complement. Codons > 50 = gene (in prokaryotes: orf finding (website) Ncbi orf, etc: cons - false positives, gene not simple to find, overlook small genes, over predict. When present in water - repels h2o by aggregating together, resulting in a strong force: sense/forward strand 5" to 3", antisense/complement strand 3" to 5", fundamental paradigm is dna to rna to protein. If no ribosome bound, will degrade: stem-loop terminators (rho independent) Self-complement, forces ribosome to fall off: high gc content (cpg islands) locates genes in genomes, **ideally will need, pssm, hmm, web tools for prokaryotic gene identification.