BIOL107 Lecture Notes - Lecture 14: Atp Synthase, Cytochrome C Oxidase, Intermembrane Space

• School: University of Alberta
• Department: Biology (Biological Sciences)
• Course: BIOL107
• Professor: A L L

Document Summary

Complex iii, etc: oligomyocin, inhibits the flow of h+ through atp synthase therefore no atp made, addition of oligomyocin to cells prevent dissipation of h+ gradient; electrons flow through complexes i, ii, iii and h+ are still pumped, h+ concentration on intermembrane space side of the mitochondrial inner membrane becomes very high. When this concentration gets high enough, inhibition of electron transfer and h+ pumping reactions of complexes i, ii, iii therefore, these activities stop: dinitrophenol (dnp): makes mitochondrial inner membrane permeable to h, enables equilibrium of gradient, no h+ gradient forms so there is no flow back through atp synthase. No atp made: electron transport continues, dnp uncouples electron transport and formation of the h+ gradient, uncoupler, molecules other than glucose can contribute directly to atp production, all foods can be used as sources of energy when broken down by enzymes into molecules that enter glycolysis or the krebs"s cycle, ref fig.