amber_michal_121022.pdf

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Department
Biology (Biological Sciences)
Course
BIOL201
Professor
Michael Harrington
Semester
Fall

Description
PAL  Worksheet   Week  of  October  22 ,  2012   nd Meiotic  Recombination  Mapping     1.    You  are  a  crazy  cat  person  (which  means  you  &  I  would  get  along  great).    You   want  to  investigate  if  the  autosomal  loci  for  blue  eyes  (B)  and  big  ears  (E)  are  linked.     You  cross  true  breeding  blue-­‐eyed  cats  with  big  ears  to  true  breeding  green-­‐eyed   cats  with  little  eyes.    You  then  cross  the  F1  progeny  with  a  tester  strain  &  observe   the  results  below:   Genotype   Phenotype   #  observed   Parental  or  recombinant?   B/b•E/e   Blue  eyed,  large  ears   60     B/b•e/e   Blue  eyed,  small  ears   13     b/b•E/e   Green  eyed,  large  ears   60     b/b•e/e   Green  eyed,  small  ears   17         Calculate  the  recombination  frequency  between  the  blue  eyes  and  big  ears  loci  &  the   distance  between  the  two  genes.             2.    Continuing  on  with  cats,  the  claw  locus  controls  claw  size  and  long  (L)  is   dominant  to  short  (l).    You  cross  a  true  breeding  blue  eyed  cat  with  short  claws  to  a   true  breeding  green  eyed  cat  with  long  claws.    You  then  cross  the  F1  with  a  tester.     a. If  the  claw  locus  is  36  map  units  from  the  blue  eyes  locus,  what  proportion  of   F2  progeny  will  be  green  eyed  with  short  claws?                  
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