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University of Alberta
Biology (Biological Sciences)
Michael Harrington

Biology 207 - Practice Questions 3 - Answers Question 1 What is the purpose of: See me if you want your answers checked. Question 2 Consider the experiment in Lecture 5 section 4c. a) The symbol '+' is used to represent three different things. What are they? A functional, wild type allele. Growth of the cells. And. b) Positive and negative controls are done so that we know that an experiment has worked the way it should. Which control(s) in the table allow us to know that we didn't make the following mistakes? Question 3 Students often confuse biochemical pathway analysis with complementation tests. To illustrate the differences consider this synthesis pathway from S. cerevisiae. You have isolated three mutant strains unable to synthesize compound “B”. The blocked step in each mutant strain is shown. Mutations 1 and 3 are in the same gene. Complete both tables with the expected results where + indicates growth and - indicates no growth. Also identify positive controls and negative controls. a) Biochemical pathway analysis results b) Complementation test results Question 4 You have isolated five Neurospora mutant strains that are unable to synthesize an essential compound, C. In order to determine the biochemical pathway that synthesizes C you tested each of the five mutant lines for their ability to grow on minimal media (MM) and minimal media supplemented with compounds D, E, and C. (Compounds D and E are similar in structure to compound C.) The results of growth (+) or no growth (-) were: Strain MM MM+D MM+E MM+C mutant 1 -
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