BIOL201 Lecture Notes - Intron, Escherichia Coli, Auxotrophy
11 Apr 2014
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Biology 207 - practice questions 4 - mutations. Question 1 the a-gene makes a pre-mrna with one intron. Make a diagram showing how the pre-mrna is processed normally and what might happen if there is a mutation in the exon/intron boundary. Question 2 recall that ems causes gc-->at bp substitutions. Use a diagram to show why or why not. Question 3 the laci gene in e. coli has mutational hot spots. Hot spots are sites in a gene that are much more mutable than other sites. The following sequence is from the middle of the wildtype laci gene: Question 4 these petri plates were inoculated with 109 salmonella cells. The cells are auxotrophic for histidine and the plates are minimal media. In the centre of the right plate there is a disk saturated with a mutagen. Describe the patterns of colonies found on both plates.
