Class Notes (837,001)
Canada (509,985)
BIOL207 (121)
Lecture 8

BIOL 207 (09/27/13) Lecture 8: Usefulness of Gene Mutations | Lecture 9: Mendel Invents Genetics

4 Pages
Unlock Document

Biology (Biological Sciences)
Mike Harrington

BIOL 207 (September 27, 2013) Lecture 8: Usefulness of Gene Mutations | Lecture 9: Mendel Invents Genetics How do Geneticists Obtain Mutations  Spontaneous mutations? o Too rare (usually)  Exception: Drosophila  Mutant Screens? o Induce a lot of mutations and isolate individuals with desired mutation o Problem: Screening in multicellular organisms is A LOT more DIFFICULT than screening unicellular organisms  Good for bacteria though (ex. Screening auxotrophs of Neurospora) o Steps for screening of Drosophila:  1 – Expose male flies to mutagen through EMS in food or x-ray  EMS  point mutations  X-rays  deletions  Somatic mutations are interesting, but not what we want for the purpose of screening because the mutation dies with the individual… we’re interested in the germ line mutations here  Mate flies  Males have mutations that are both somatic AND germline o Why males?  So only one mutation is passed onto the offspring  Male flies can produce more offspring  Collect mutant flies  Individuals with desired phenotype are collect and made into a permanent stock while the others are discarded  Characterize mutant strains (via experimentation)  Targeted Gene Knockout? o Replace WT allele with mutant one o Example: Mice  Mutant strains lacked telomerase RNA  Result? DNA started losing telomeres and fusion of chromosomes with telomeres only on one side occurred (to make it so that there are telomeres on both ends) o Protein’s way of “fixing” this mutation  With enough time and money, you can create a mouse with any mutation I-Clicker Question: Q: You have isolated five mutant strains of Drosophila that all have the same mutant phenotype. How many different genes have you identified
More Less

Related notes for BIOL207

Log In


Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.