CELL201 Lecture Notes - Lecture 12: Tubulin, Non-Covalent Interactions, Mreb

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Major structural elements of the cytoskeleton: microtubules, microfilaments. Polar (+ and ends) (+) and (-) ends: cytoskeletal system of bacteria. Mreb- actin like protein involved in dna segregation. Ftrz- tubulin like protein involved in regulating division. Two types of microtubules: cytoplasmic microtubules and axonemal microtubules. Cytoplasmic: movement of vesicles, formation of spindles and maintaining or altering cell shape. Protein building blocks of microtubules: tubulin heterodimers (non-covalent bonding) A and b subunits have similar structures but only 40% amino acids are identical. N-terminal binds to gtp, c terminal associated with maps and central domain can bind to colchicine. In the presence of gtp and mg2+ reversible polymerisation of tubulin dimers take place. Elongation is addition of dimers to either end. The tubulin concentration at which mt assembly is exactly balanced by disassembly is the critical concentration. Addition of tubulin dimers occur more rapidly at the plus end.

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