IMIN200 Lecture Notes - Lecture 6: Capsid, Poliovirus, Reverse Transcriptase

• School: University of Alberta
• Department: Immunology and Infection
• Course: IMIN200
• Professor: Kathy Magor

Document Summary

Concerted assembly - proteins themselves have w/i them; new biochemistry forms capsid automatically (occurs by itself). Virus attaches to pvr > attachment opens capsid. Once capsid is open, rna gets out. Rna is already in (+) strand orientation can encode protein. 1st proteins: translated into one long polyprotein: p1 - capsid, p2 + p3 - enzymes nec for translation. Initial cleavage is autocatalyzed, but proteases are released. On outside surface of vacuole -> structural place for proteins that bring over rna into protein and attach. 3ab + 3c poly : bring over 1st rna strand > make more copies of it ( - strand) (+) strand: makes (-) strand using rna-dep rna poly. Rna: cleavage of last protein has enzymatic activity & works as a protease from inside = stabilize capsid. Already in a form needed to assemble > auto assemble once certain conc is reached. Each capsid have to get rna strand in.