IMIN405 Lecture Notes - Lecture 4: Intrinsically Disordered Proteins, Zap70, Phospholipid

• School: University of Alberta
• Department: Immunology and Infection
• Course: IMIN405
• Professor: James Stafford

Document Summary

Lecture 4: immunoregulation: overview of immunoregulaiton, fundamentals of signal transduction, tyrosine-based signaling motifs: itams and itims, key players: src and syk, phagocytosis as a model for understanding immunoregulatory receptor-mediated. Primary effector, secondary effector, then more and more: signal transformation and signaling events amplification. Localization: if one move to membran eo nucleus it would serve diff purposes. Stimulatory receptor types that have charged transmembrane residues that allows them to inteact with adaptors, negatively charegd aa. Itam: tyrosines separated by 14 aa embedded in a motif, they become sites of phosphorylation. Sh2 domains: aa sequences that recognized phosphorylated tyrosines. Sh2 domains are looking for phosphotyrosine in a very specific motif. Shp-1 has affinity in an itim, selective recruitment. Sh2 and tbh bind phosphorylated tyr motifs. Kinases: can delivery more activation or signaling. General strcuturure of sh2 domain: antiparallel b strands, alpha helices. Binding pocket for tyrosine phosph: contact residues. Multiple sh2 domains; different proteins for their targets.