IMIN200 Lecture Notes - Mhc Class I, Antigen-Presenting Cell, Mhc Class Ii

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sangriaseal840

10 Apr 2014

School

University of Alberta

Department

Immunology and Infection

Course

IMIN200

Professor

All

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Document Summary

After studying lecture #7, you should be familiar with the following concepts: antigen presenting cells (apcs). Professional apcs (e. g. dendritic cells, macrophages) capture microbial protein antigens from the skin, gastrointestinal tract, respiratory tract and the bloodstream. Apcs process these antigens intro a diverse number of peptides and present them to t cells in lymph nodes. Presented antigens provide the first signal for t cell activation. Apcs that have encountered microbial agents also express costimulators on their surface. These costimulators provide the second signal required for t cell activation. Apcs also express cytokines that direct the t cell response: the major histocompatability complex (mhc). Apcs present antigens on mhc class i or mhc class ii molecules. Each mammal encodes multiple mhc i and mhc ii molecules and each mhc allele is highly polymorphic. In addition, each mhc molecule can bind and present a large number of peptide antigens.

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Question 4 (9 points):

To study T cell development, and self-restriction of T cells, you decide to perform some experiments. You obtain four mouse strains: B, B-MCC, K, and K-MCC. B strain mice express MHC molecules of the b haplotype (e.g. H-2K^b, H-2D^b, I-A^b) while K mice express MHC of the k haplotypes (e.g. H-2K^k, H-2D^k, I-A^k). The strains are otherwise genetically identical. B-MCC and K-MCC are identical to the B and K strains (respectively) excepts that contain a transgene encoding the antigen Moth Cytochrome which is not present in normal mice. MCC is produced as both a secreted and cytoplasmic protein and it is expressed by dendritic cells and thymic epithelial cells. You lethally irradiate groups of mice from all four strains and perform all possible bone marrow transfers among the mice. In other words, the BM of each strain is transplanted into mice of each strain, producing 16 different combinations of donor BM and recipient strain. You now take T cells from the lymph nodes of each of the 16 types of transplanted mice and test their ability to proliferate in response to MCC-presenting B cells that express only class II MHC molecule, I-A^k. Fill out the chart below by indicated which mice will have T cells that respond. Use (+) to indicated proliferation and NR to indicate a lack of proliferation. In addition, for the mice labelled a-e give a brief explanation for your answer. Include the mechanism of T cell selection experienced in thymus (positive selection, negative selection, or neglect) and the cells involved in selection (thymic epithelial cels, BM-derived APCs, or both).

Table (4 points, 0.25 points per box)

Recipient Strain Â®

BM Donor	B	B-MCC	K	K-MCC
B			c.
B-MCC			d.
K	a.	b.	e.
K-MCC

1 point for each explanation

yellowguinea-pig868

How did inoculation with cowpox generate immunological memory and provide protection against smallpox? Did cross-reactivity play a role?

How did Pasteurâs work with attenuated (weakened) bacteria further our understanding of the role vaccination can play?

What are the three characteristics that define an adaptive immune response? Briefly define each of the characteristics.

Define primary versus secondary immune response.

Whatâs the difference between totipotent, pluripotent, multipotent and oligopotent when evaluating cell differentiation capacity?

What is hematopoiesis?

What cell types are derived from myeloid versus lymphoid progenitor cells? Describe the function of each WBC type.

What is clonal selection?

What role do antibodies play with regard to antigen recognition and immune defense that is different from what Tcrs provide?

As described in class generally understand the outcome of B cell, Th cell, and Tc cell activation. Understand that these cells can differentiate into effector (plasma cells, cytokine producing Th cells and CTLs) or memory cells.

Understand how granzymes and perforins play a role in apoptosis.

Which types of cells typically express MHC Class II? Do these cell types also express MHC Class I, and if so why?

With regard to Tc and Th cells, what kinds of peptide antigens (think about exogenously versus endogenously derived antigens) are recognized?

Be able to show cooperation between Tc, Th and B cell responses (refer to class notes).

What is the overall role of MHC Class I and II?

What is ADCC and what cell types are involved?

Understand and be able to provide examples highlighting the cooperation between innate and adaptive immunity. Note: ADCC and dendritic cell and macrophage interactions with Th cells illustrate examples of innate/adaptive cooperation.

What are the primary lymphoid organs? What role do they play?

What role does the lymphatic circulatory system play in immune defense?

Whatâs the difference between plasma, ISF, and lymph?

Whatâs the difference between afferent and efferent lymphatic vessels?

How is lymph ultimately delivered back to blood?

Define extravasation. Why is this important?

What are HEVs and what role do they play?

What are the secondary lymphoid tissues/organs?

What role do lymph nodes play?

What role does the spleen play?

What is Leukocyte Adhesion Deficiency?

Whatâs MALT and its role?

What is the inflammatory response and what are characteristics associated with inflammation?

What role does histamine play in the inflammatory response?

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IMIN200 Lecture Notes - Mhc Class I, Antigen-Presenting Cell, Mhc Class Ii

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