MICRB265 Lecture Notes - Lecture 26: Contig, Consensus Sequence, Lactobacillus
23 Dec 2017
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Dna sequencing uses sanger sequencing method; 1st generation. previous methods. Read lengths 700 to 900 base pairs. 1977 fred sanger utilized this method which is more accurate, and easier than. Dntp is deoxynucleotides. and ddntp is dideoxynucleotides. The analog is a chain terminator because it lacks 3" hydroxyl! Reminder; dna synthesis is from 3" to 5" always. Dna sequencing using pyrosequencing method 2nd generation. Reads length between 400 to 500 base pairs. Similar to first generation; you have template, dna polymerase and you are synthesizing. Dna strands. the differences are; instead of synthesising one strand at a time, they can synthesis thousands of strands in parallel. Dna is cleaved into fragments using restriction endonuclease enzymes. a consensus sequence for previously unknown dna sequences. because it is a continuous sequence). Fragments are then sequenced, and a program finds overlaps and orders fragments into. Consensus sequence is a sequence that is contained in every fragment. (called a contig.
