MICRB265 Lecture Notes - Lecture 8: Human Microbiota, Yeast, Acetoin

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scarletjellyfish828

25 Apr 2018

School

University of Alberta

Department

Microbiology (Biological Sciences)

Course

MICRB265

Professor

Perrin Beatty

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Document Summary

Nutritional diversity: carbon and energy sources: definition of prefixes, energy sources: phototrophs use light energy plus an electron source (e. g. , h2o or h2s, oxygenic photosynthesis splits water to release o2, anoxygenic photosynthesis uses other electron sources e. g. h2s. S (elemental sulfur) and does not release o2 e. g. thiomargarita namibiensis, thiospirillum jenense chemotrophs use chemical energy. The energy source prefix is named first, then the c source prefix. Note that the terms below are simplified compared with those used in the textbook. Photoautotrophs: use light energy to fix co2 as their carbon source; e. g. most photosynthetic organisms, including cyanobacteria, algae, green plants. Photoheterotrophs: use light energy and organic compounds as c source. Chemoautotrophs: use chemical energy (redox reactions using inorganic or organic compounds) to fix co2 as their c source. Chemoheterotrophs: use chemical energy (redox reactions using inorganic or organic compounds) to assimilate organic c as carbon source. These are the most common of the organisms that we study in the lab.

Related Questions

Phototrophs need an electron source like H₂S orH₂O. Where do the electrons from these sources go?(Assume the organism is growing autotrophically as well astophototrophically.)

	O₂ and CO₂
	O₂ only
	CO₂ only
	Sulfate or oxygen
	Inorganic various molecules, depending on cellular needs.

Which of the following statements about chlorophyll istrue?

	It is a good electron acceptor in its oxidized form.
	It is a good electron donor in its reduced form when energizedby light.
	It contains magnesium.
	It is a tetrapyrrole.
	All of the above

The unifying feature of all photosynthetic organisms isa(n)

	electron transfer chain.
	electron donating system to generate NADPH.
	ATP-generating system.
	light-gathering system.
	All of the above

Evidence suggests that banded iron formations are due to

	chemical oxidation of reduced iron, Fe²⁺.
	aerobic reduction of insoluble ferric iron, Fe(OH)₃.
	anaerobic oxidation of soluble ferrous iron,Fe²⁺.
	Both a and b
	Both a and c

In addition to their ability to harvest light in the 400 to 500nm range, carotenoids also

	eject electrons from the reaction center.
	function as a tetrapyrrole.
	intercept toxic single-state oxygen.
	transfer electrons to ubiquinone.
	raise electrons to a higher energy state.

Ribosomal RNA and other cell features affirm that living cellsshould be divided into three groups called domains.

	True
	False

Microbes have started to evolve recently.

	True
	False

The ________ was essential for the evolution of oxygenicphotosynthesis.

	evolution of cyanobacteria
	development of photosystem I
	ability to extract electrons from CO₂
	ability to extract electrons from H₂O
	ability to use oxygen as the terminal electron acceptor

What do oxygenic phototrophs use as an electron source?

	Oxygen
	Sulfide
	Water
	Organic compounds
	CO₂

10.

Three domains of organisms have been established basedprimarily on size and other structural features of the cells.

	True
	False

fuchsiasea-lion54

1. Plants need which of the following to carry on photosynthesis?

a. H2O

b. CO2

c. O2

d. both O2 and CO2

e. both H2O and CO2

2. Organisms that derive their chemical energy either from the process of chemosynthesis or photosynthesis are classified as

a. autotrophs.

b. parasites.

c. heterotrophs.

d. saprophytes.

e. mutualists.

3. The carbon source for organisms that derive their energy from photosynthesis is

a. carbon monoxide.

b. carbon dioxide.

c. hydrocarbons.

d. methane.

e. glucose.

4. The oxygen released in photosynthesis comes from

a. carbon dioxide.

b. glucose.

c. ribulose bisphosphate.

d. water.

5. When chlorophyll a molecules absorb energy, the molecules

a. release electrons.

b. release X-rays.

c. release energy.

d. release electrons and X-rays.

e. release electrons and energy.

6. The flow of what particle across the thylakoid membrane powers the production of ATP?

a. electrons

b. hydrogen ions

c. oxygen

d. carbon dioxide

e. phosphate ions 2

7. The light-independent reactions were discovered by

a. M. D. Hatch.

b. Andrew Benson.

c. Melvin Calvin.

d. Robert Hill.

e. both Andrew Benson and Melvin Calvin.

8. For each six atoms of carbon dioxide fixed in the light-independent reactions, how many molecules of PGAL (phosphoglyceraldehyde) are produced?

a. 2

b. 3

c. 6

d. 12

e. 15

Chapter 7

9. The ultimate source of energy for living things is

a. the Krebs cycle.

b. fossil fuels.

c. the sun.

d. glycolysis.

e. aerobic respiration.

10. When molecules are broken apart in respiration

a. the heat produced is used to drive biological reactions.

b. the oxygen in the compounds that are broken apart is used as an energy source.

c. the energy released in respiration is channeled into molecules of ATP.

d. ATP is converted into ADP.

e. ADP is released as a waste product.

11. ATP

a. can be produced by photosynthesis.

b. is produced in the degradation of organic compounds such as glucose.

c. is generated in anaerobic respiration.

d. is released in aerobic respiration.

e. all of these

12. Which part of the energy-releasing pathway can convert all carbon that enters to carbon dioxide?

a. fermentation

b. glycolysis

c. Krebs cycle

d. electron transport

e. phosphorylation

13. Which liberates the most energy in the form of ATP?

a. aerobic respiration

b. anaerobic respiration

c. alcoholic fermentation

d. lactate fermentation

e. All liberate the same amount, but through different means.

14. Aerobes use ________ as the final electron acceptor in electron transport phosphorylation.

a. hydrogen

b. carbon

c. oxygen

d. H2O 3

15. Glycolysis depends on a continuous supply of

a. NADP.

b. pyruvate.

c. NAD+.

d. NADH.

e. H2O.

16. Glycolysis

a. occurs in the mitochondria.

b. results in the production of pyruvate.

c. occurs in the cytoplasm.

d. occurs in the mitochondria and results in the production of pyruvate.

e. results in the production of pyruvate and occurs in the cytoplasm.

17. The end product(s) of glycolysis is(are)

a. acetyl CoA.

b. carbon dioxide.

c. pyruvate.

d. glucose.

e. glucose and carbon dioxide.

18. Which is capable of being reduced during both glycolysis and the Krebs cycle?

a. NAD+

b. FAD+

c. ADP

d. NADH

e. NADP+

19. The Krebs cycle takes place in the

a. ribosomes.

b. cytoplasm.

c. nucleus.

d. mitochondria.

e. chloroplasts.

20. The breakdown of pyruvate in the Krebs cycle results in the release of

a. energy.

b. carbon dioxide.

c. oxygen.

d. energy and oxygen.

e. energy and carbon dioxide.

ochremole959

5. Some bacteria live in deep sea vents where no light penetrates, yet are still capable of producing their own reduced carbon (e.g. fixing carbon dioxide). These organisms are classified as

(A) photoheterotroph (B) photoautotroph

(C)chemoheterotroph (D)chemoautotroph

(E) organotroph

6. Nitrogen fixation is the conversion of

(A) ammonium ion to nitrogen gas. (B) nitrogen gas to ammonium ion.

(C)nitrate ion to nitrogen gas. (D)ammonium ion to nitrate ion.

(E) nitrate ion to ammonium ion.

7. Which of the following is true of complex media?

(A) Bacteria grow faster on them than on synthetic media.

(B) All the chemical components and their concentrations are known.

(C)Complex media are the most difficult of all to prepare.

(D)Complex media can help determine the metabolic capacity of an organism.

(E) They are also called minimal media.

8. Which of the following types of growth media contains blood?

(A) minimal media (B) complex media

(C)defined media (D)enriched media

(E) selective media

9. The bile salts and crystal violet in MacConkey lactose plates prevent the growth of Gram-positives. These additives make the MacConkey plate a

(A) complex medium. (B) defined medium.

(C)minimal medium. (D)selective medium.

(E) differential medium.

10. You discover a new bacterial efflux pump that moves an uncharged toxin out of the cell using the proton motive force as the energy source. This new transporter can be classified as an

(A) electrogenic antiporter (B) electrogenic symporter

(C)electroneutral antiporter (D)electroneutral symporter

(E) none of the above

11. Which of the following involves nitrate as a terminal electron acceptor?

(A) photosynthesis (B) aerobic respiration

(C)fermentation (D)anaerobic respiration

(E) glycolysis

12. Which of the following is least likely to use glucose (an organic molecule) as a source of electrons?

(A) lithotrophy (B) organotrophy

(C)aerobic respiration (D)anaerobic respiration

(E) heterotrophy

17. Which of the following nutrient uptake systems does not require cellular energy?

(A) facilitated diffusion (B) coupled transport

(C)ABC transporters (D)group translocation

(E) All of the above require energy expenditure by the cell

18. What is the energy source for ABC transporters?

(A) proton motive force (B) NADH

(C)phosphoenolpyruvate (PEP) (D)ATP

(E) No energy is required for ABC transport.

19. Which of the following is true of all biofilms?

(A) They only contain a single species of bacteria.

(B) Biofilms can form on a range of biotic or abiotic surfaces.

(C)Cells in a biofilm are dormant until the bacteria leave the biofilm.

(D)Biofilms are composed of a single monolayer of EPS.

(E) Biofilms are not found in or on the human body.

20. The heterocyst cells of cyanobacteria are specialized for

(A) photosynthesis (B) gliding motility

(C)nitrogen fixation (D)carbon fixation

(E) antibiotic production

MICRB265 Lecture Notes - Lecture 8: Human Microbiota, Yeast, Acetoin

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