MICRB315 Lecture Notes - Lecture 4: De Novo Synthesis, Stainless Steel, Biotransformation
18 Oct 2015
School
Department
Course
Professor
Document Summary
Difficult to make fermenter with glass in first place. Temperature (difficult for sterilization, dissipation of heat) Potential for deterioration (scratches -> place for contaminants) Plastic - heat and conductivity, etc. and esp downstream processes that involve organic materials (since plastic is also made from organic chemicals - will ferment it itself) Works well with small scale though gold coating: Production of primary metabolites at industrial scales: outline. Carboxylic acids a) citric acid (aerobic fermentation) b) lactic acid (anaerobic fermentation) Amino acids a) glutamate b) lysine: (i) biotransformation (ii)!direct!fermenta. on! Vanillin flavouring a) microbial biotransformation de novo synthesis in yeast b) Total world market . 5 billion (2004) big world market some aa are cheaper made by other processes, not microbial. Competes with chemical syntheses for some amino acids. Direct fermentations of glutamate, lysine, arginine, and phenylalanine + aspartate (to make aspartame) Transformations by immobilized cells or enzymes not actually producing aa -> taking chemically synthesize and use microbes to alter it.
