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Lecture

MICRB 265 (09/09/13) - Glycobiology Guest Lecture By Dr. Christine Szymanski

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Department
Microbiology (Biological Sciences)
Course Code
MICRB265
Professor
Brian Lanoil

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MICRB 265: Guest Lecture (September 9,2013)
Dr. Christine Szymanski - Glycobiology
Macromolecules include:
o Proteins
o Carbohydrates
o Lipids
o Nucleic acids (DNA/RNA)
FUN FACT: Clownfish have the ability to live within the tentacles of sea
anemone without being stung. How so? Clownfish actually have a dense layer
of sugar coating them and this sugar coating insulates them from the sea
anemone’s stings.
Gram-positive microbes have a very thick peptidoglycan layer as compared
to the gram-negative counterparts.
o When gram-stained, gram-positive microbes appear purple and gram-
negative microbes appear pink (from safranin)
GLYCOBIOLOGY
Is the study of the structure, biosynthesis (how organisms produce), biology,
and evolution of saccharides
o Saccharides include sugar chains and glycans
Rapidly growing field in the natural sciences
Campylobacter Jejuni (C. Jejuni)
Bacteria that dwells in feces… especially from poultry
Gram-negative
Antibiotics are effective against this bacteria
o If untreated though, victims may become paralyzed or die
o C. Jejuni can induce Guillain-Barre Syndrome
Causes auto-immune attack
NEED sugar in order to develop flagella
Capsular Polysaccharides
Protect bacteria from the environment and provide cell membrane structural
support
Sugars in cytoplasm added to something (eg. lipid) and pushed across the
membrane through a channel to the outside of the cell
Glycosylation enzymatic process where sugars are linked together to produce

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Description
MICRB 265: Guest Lecture (September 9,2013) Dr. Christine Szymanski - Glycobiology  Macromolecules include: o Proteins o Carbohydrates o Lipids o Nucleic acids (DNA/RNA)  FUN FACT: Clownfish have the ability to live within the tentacles of sea anemone without being stung. How so? Clownfish actually have a dense layer of sugar coating them and this sugar coating insulates them from the sea anemone’s stings.  Gram-positive microbes have a very thick peptidoglycan layer as compared to the gram-negative counterparts. o When gram-stained, gram-positive microbes appear purple and gram- negative microbes appear pink (from safranin) GLYCOBIOLOGY  Is the study of the structure, biosynthesis (how organisms produce), biology, and evolution of saccharides o Saccharides include sugar chains and glycans  Rapidly growing field in the natural sciences Campylobacter Jejuni (C. Jejuni)  Bacteria that dwells in feces… especially from poultry  Gram-negative  Antibiotics are effective against this bacteria o If untreated though, victims may become paralyzed or die
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