MICRB316 Lecture 6: Lecture 6.doc

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Same degree of dna melting occurs after some elongation as at transcription initiation. But, the static nature of diagrams is misleading: How does elongation procede: rotation of rnap around dna template, rna is twisted around the dna strand c. Rnap moves in a straight line / dna rotates. Template rotates in one direction to unwind, rotates in the other to wind up again. Unwinding caused by the advancing polymerase causes a compensationg overwinding ahead of the unwound region. Mutations in topoisomerases that relax either type of supercoils, acculmulate these supercoils. Because: dna polymerase may be immobilized and, there is some homology between all polymerases; Suggested that dna polymerases might be attached to the bacterial membrane to facilitate control over. Cells were briefly treated with radiolabelled nucleotides, broken open, then treated with nuclease to detach most dna. Results: the newly made dna was still attached to the cell wall and membrane in bacteria. But now, this is still considered a possibility.

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