MMI133 Lecture Notes - Lecture 1: Phospholipid, Peptide, Safranin

• School: University of Alberta
• Department: Medical Microbiology and Immunology
• Course: MMI133
• Professor: Judy Gnarpe

Document Summary

Bacteria is classified into a family based on genetic information. When naming the genus - always use uppercase and italicize. When naming the species - use lowercase and italicize. Fixing the specimen: before applying any stains, the bacteria must be fixed which means it must be able to stick to the plate so when stains and washes are applied, the bacteria will not fall off a. This can be done with direct heat or methanol. Methyl violet wash: methyl violet is a dark purple stain that will penetrate the walls of the bacteria and stain all the bacteria (regardless of their gram status) a dark purple colour. This large molecule is difficult to get out. Rinse with acetone or alcohol: this will completely wash everything off (excess methyl violet and iodine), in gram negative cells it will wash out the large molecule inside this bacteria a. b.