NU FS363 Lecture Notes - Lecture 24: Fecal Coliform, Indicator Organism, Listeria Monocytogenes
9 Aug 2017
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Culture based methods stand out, quite sensitive, culture step/plating method. Culture based is not that specificity (difficult to distinguish different strains of e. coli) Detection of bacteria in food: criteria for assay selection. Cost (less time spent in lab means less expensive) Listeria monocytogenes in vacuum packaged ham: negative per package (200g) Enterohaemorrhagic e. coli in ground beef: negative in 375g. Official methods published by health canada in the compendium of analytical methods. Compendium of analytical methods validated in inter-laboratory studies (accuracy and precision) Ready to eat products, need to have negative results in all. In bacteriology, viability is equated to culturability plate count or most probable number as indicator for viable cell count (selective and/or elective media) If you (cid:272)a(cid:374)"t (cid:272)ulture it, that (cid:373)ea(cid:374)s (cid:374)ot ali(cid:448)e. Culture methods are limited to identification on genus or species level. Need some dna methods, it is not possible to differentiate to species level with only culture based methods.
