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Lecture

Lec30_140326.pdf

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Department
Statistics
Course
STAT151
Professor
Gregory Wagner
Semester
Winter

Description
Lec30 BIOL207 2014-03-26 CHAPTER 11 Genomics A. Genomics (and other ‘omics technologies) a. Study many (>100s) of different molecules in parallel b. Genomics: study of complete (or nearly complete) set of genes in an organism i. Transcriptomics = complete set of transcripts ii. Proteomics = complete set of proteins iii. Metabolomics = complete set of metabolites B. DNA sequencing a. How do you sequence a DNA molecule i. Sanger invented dideoxy sequencing = chain terminator sequencing; widely used since mid-1980s ii. Relies on ddNTPs, which lack 3’OH to which next base is added during DNA polymerization iii. Reaction includes normal dNTPs, plus ddNTPs that are fluorescently labeled (each base has a different color of label), primer, polymerase iv. Denature template, anneal pri mer, allow polymerase to extend growing strand v. Elongation will terminate whenever a ddNTP is incorporated vi. Separate all of the terminated fragments by electrophoresis vii. Reading from the bottom of the gel, color of each band will tell you the sequence of the DNA viii. Can read up to 700bp of DNA from millions of copies of the same gene in a single reaction
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