ZOOL342 Lecture Notes - Lecture 4: Electrophysiology, Membrane Potential, Voltage Clamp

• School: University of Alberta
• Department: Zoology (Biological Sciences)
• Course: ZOOL342
• Professor: Declan Ali

Document Summary

The relative permeability of potassium is one, and na and cl is lower than 1. Cl follows potassium, and membrane potential depends on chloride. Chloride we ip in the equation since it has a negative charge: ex. calculation: Vm= 58 log pk[k]o + pna[na]o + pcl[cl]i/ pk[k]i + pna[na]i + pcl[cl]o. = 58 log 1[5] + 0. 04[130] + 0. 45[4] / 1[150] + 0. 04[10] + 0. 45[140] The pump is always working in low levels the cells are at rest. When working 1 net of negative charge out of the cell. Requires atp, the pump has a intrinsic atpase. Na+/k+ transporter: 2 potassium and 3 na is exchanged for every atp hydrolysed. At 20min add li, and nothing happens so the pumps do not require li and the pump is speci c to na only. Adding na second time, to see if it happens again.