ZOOL344 Lecture Notes - Lecture 5: Glucose Transporter, Electrochemical Gradient, Lipid Bilayer
15 Nov 2017
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This is followed by a conformational change to inward-facing sites: glucose and na+ dissociates form their binding sites (either na+ or glucose first) into the cytosol. This allows the protein to revert to its original outward-facing conformation. Note: since this is coupled to na+/k+ atpase, this is known as secondary active transport. In some fish species, some absorption also occurs: midgut, components: small intestine, functions, the midgut is the principal site of digestion of proteins, carbs, and lipids. Impairing the delivery/availability of the ligand of interest and/or abolition of key molecules in the target tissue/cell negatively impact the physiological function(s) of interest. Immunoprecipitate proteins with one antibody (this should pull out any other proteins associate with this first protein molecules), run a western as above using another primary antibody against the protein that should be associated with the first one.
