CMMB 411 Lecture Notes - Lecture 17: Uridine, Pseudouridine, Hydrogen Bond
18 Nov 2018
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Document Summary
Majority of unusual nucleotides ( >50) are found in trnas. In biological systems for rna, there are a total of 150 modifications for trna, mrna, rrna combined. These modifications are generated post-transcriptionally, and therefore are known as post- transcriptional modifications. Modifications are found in all 4 bases. More complicated modifications are found in purines (ag) Some modifications are common in all trnas (eg. d and ) and are universal. Compared with regular uridine (u), the major difference lies in c5 (carbon 5) and c6. These two carbons are connected by a double bond in the regular, unmodified uridine molecule. After a reduction process, you introduce 2 extra hydrogen molecules and reduce the double bond to a single bond. Despite the modification, there is still hydrogen bonding with the a nucleotide to the same degree. Try to avoid loops in stem region.
