CMMB 411 Lecture Notes - Lecture 16: Kozak Consensus Sequence, Start Codon, Cistron

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Start codon: aug >> gug > uug (bacteria) > methionine (met) For bacterial cells, there can be 3 different start codons. Most common start codon is aug, least common is uug. Gug normally codes for valine, uug codes for leucine. If these codons right now function for initiation purpose, they will insert methionine instead of the other amino acids they may code for. When they are not involved in coding, see these codons everywhere. Common but not present in all eukaryotic mrna. Sequence is commonly present in mrna, but not always there. If sequence is there, it will follow the rules of the kozak sequence to determine which aug to select for initiation. Nucleotide at +4 position can inference the efficiency of translation. -3 site is always located upstream of the aug codon. Nucleotide at this position is more important than the nucleotide at the +4 position. Apply 5 rules to determine which is good initiation codon.

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