CMMB 413 Lecture 3: The Principles of Clinical Cytogenetics — Part 2
21 Feb 2019
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The principles of clinical cytogenetics part 2. Small changes in chromosome structure detected by in site hybridization. Large changes in chromosome structure detected by banding. When chromosomes undergo condensation, the accessibility of dyes to bind to dnas is different, as a result, need to use different staining methods and dyes to see the changes in chromosome structure better. Used to detect heteromorphism, a morphological variant of chromosome. Allows us to distinguish heteromorphism and homologous chromosomes. Is a slight morphological difference between 2 homologous chromosomes. Use a dye to stain metaphase chromosome. Is the standard method used in europe. The resulting dark and light bands are the reverse of g and q banding methods. Used to analyze the distal ends of chromosomes that stain poorly with g or q banding methods. In g banding, dark band is at rich. Reverse of what happens in g banding, therefore it is called reverse banding. Tries to distinguish between distal ends of chromosomes.
