ANSC 4470 Lecture Notes - Lecture 10: Protein Kinase B, Phosphorylation, Pyruvic Acid

• School: University of Guelph
• Department: Animal Science
• Course: ANSC 4470
• Professor: John Cant

Document Summary

Animal #2 was more sensiive (had to infuse more glucose into animal #2) Large insulin number to ensure signiicant increase in insulin levels in animal (e. g. 60 mu), big enough change in the animal. Give bolus of insulin, get up to plateau quickly: measure glucose lux in animal (at basal level and ater infusing insulin) Total lux = gir (glucose infusion rate) + egp. Measure before the clamp, then measure ater the clamp. Ater insulin infusion, egp should drop (insulin will suppress egp) Requires more glucose to be infused because more is being used (because egp is down) Gir is glucose uilizaion, egp is glucose producion. There are four people on one end of a bridge. They want to get to the other side, but they only have 17 minutes to do it. The bridge is weak & can hold at most two people at a ime.