BIOC 2580 Lecture Notes - Lecture 5: Edman Degradation, Frederick Sanger, Sanger Sequencing

• School: University of Guelph
• Department: Biochemistry
• Course: BIOC 2580
• Professor: John Dawson

Document Summary

Edman degration: coupling requires base; reaction must be complete before the next cyclization step can take place, cyclization requires acid; reaction must be complete before the next coupling step can take place. Trypsin and chymotrypsin convert polypeptides into smaller fragments: trypsin acts on. To yield: all fragments (except c-terminus) have phe/tyr/trp and the c-end. To yield: all fragments (except c-terminus) have hse athlete c-end. Question: how many peptides will be generated by treating the peptide below with bnbr: 3, 3, 4, 5. Cut at every m, 3 m"s = 3 cuts = 4 sections. The peptide sequences are reassembled into a complete sequence by the overlap method. Use fragments from both and assemble them so they line up perfectly. How the peptides are generated is important: when peptides are generated with trypsin - each peptide has k or r at its c-terminus, peptides generated at low ph, acidic residues have no charge on side chain; ex.