BIOC 2580 Lecture Notes - Lecture 10: Lactate Dehydrogenase, Enzyme, Enzyme Kinetics

• School: University of Guelph
• Department: Biochemistry
• Course: BIOC 2580
• Professor: John Dawson

Document Summary

Quantitative description of enzyme catalysis: rate of reaction: = concentration of substrate used time or. = concentration of product formed time (in m or um: enzyme activity : = moles substrate converted per unit time. = enzyme rate x volume: enzyme activity represents the quantity of enzyme present, si unit: 1 katal = 1 mol/s (very large unit, impractical, 1 enzyme nit = 1 umol/min = 10^-6 mol/min (widely used) 6 are stars out of 30 molecules - 6/30=20% Plot rate as a function of [s: normal chemical reactions follow simple rate laws, enzyme reaction does not follow a simple rate law. Analysis of enzyme reaction: first explored by henri in france 1905, with systematic experimental verification by michealis and. What does km tell you about an enzyme: km is the concentration of substrate [s] at which rate vo is equal to 50% of the maximum rte of.