BIOC 2580 Lecture Notes - Lecture 19: Dihydroxyacetone Phosphate, Pyruvate Dehydrogenase, Renal Medulla
26 Feb 2018
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Oxidation of glucose: complete oxidation of glucose to co2 and h2o is also accomplished in 3 stages, stage 1: glycolysis + pyruvate dehydrogenase, stage 2: tca cycle, stage 3: etc. Overview of glycolysis: occurs in the cytosol, consists of 10 different steps, the first 5 reactions - preparatory phase (atp is used to phosphorylate and activate glucose - invest. Step 1: phosphorylation of glucose: there are four different isozymes of kexokinase (i-iv). Step 2: phosphohexose isomerisation: glucose 6 phosphate and reorganize it to form fructose 6 phosphate, enzyme phosphohexo isomerase. Phosphohexose isomerase: the mechanism proceed through en ene-diol intermediate. Step 3: the second phosphorylation: phoshorylate fructose 6 phosphate at position 1 to give us fructose 1,6-bisphosphate, enzyme - phosphofructosekinease-1. To carry out glycolysis as an ongoing process the resulting nadh must be re-oxidized. This is achieved via different routes under different physiological conditions as discussed later.
