BIOC 4580 Lecture Notes - Lecture 10: Luminescence, Phase-Contrast Microscopy, Fusion Gene
22 Mar 2018
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Document Summary
Not only that, crystals might prove elusive. Since electron and x-ray crystallography are difficult and require dedicated resources, they cannot be performed by just any laboratory. Thus, bioinformatics approaches are a first tool to help understand a membrane protein(cid:495)s topology. Such predictions can be used to guide molecular and cell biological experiments to probe portions of the protein that are within the membrane, or exposed on the cytoplasmic or extracellular surfaces. These experiments involve genetic manipulation and fluorescence microscopy of transfected cells. X-ray crystallography, electron microscopy (including electron crystallography), nmr spectroscopy. Biophysical approaches for the determination of tmp structure. Modelling done through computer stimulation however must verify results experimentally. These are commonly used when studying membrane structure, large membrane protein assembly can used natural or recombinant. There is no limit to protein size and high resolution can be achieved with crystallography.
