BIOL 1020 Lecture Notes - Lecture 7: Genotoxicity, Integrase, Reporter Gene
Document Summary
Genomic sage harbors- integrated material is adequately expressed, doesn"t disturb endogenous gene structure or function (you don"t want it to ruin expression of growth hormones for example then the cow wouldn"t grow), allow precise mapping and minimizing genotoxicity. In order for viruses to survive they rely on the host resource for them to survive. They do this by inserting their genome into the host genome to survive. They don"t want to kill the host or else they will die. There is a attpsite, if they generate an attb site in the virus dna they can undergo homologous recombination, thus the virus contains integrase that allows the virus to integrate the viral gene into the host (human) By using something that is more naturally expressed, it is more accepted by the consumer as treatment (fighting mastitis in cows).
