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Lecture 3

BIOL 1020 Lecture Notes - Lecture 3: Restriction Enzyme, Endonuclease, Multiple Cloning Site


Department
Biology
Course Code
BIOL 1020
Professor
Dan
Lecture
3

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Restriction Endonucleases
Recognize specific double stranded DNA sequences and cleave at these sites. They are
proteins made in bacteria and the recognition sites are usually palindromic.
DNA in bacteria usually don’t get cut because they are Methylated, restriction endonucleases
only cut UNMETHYLATED DNA
Sticky Ends and Blunt ends
Separation of DNA- gel electrophoresis
Ori region is the DNA sequence that allows us to replicate the DNA, Multiple cloning site
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