BIOL 1020 Lecture Notes - Lecture 3: Restriction Enzyme, Endonuclease, Multiple Cloning Site
This preview shows half of the first page. to view the full 1 pages of the document.
Recognize specific double stranded DNA sequences and cleave at these sites. They are
proteins made in bacteria and the recognition sites are usually palindromic.
DNA in bacteria usually don’t get cut because they are Methylated, restriction endonucleases
only cut UNMETHYLATED DNA
Sticky Ends and Blunt ends
Separation of DNA- gel electrophoresis
Ori region is the DNA sequence that allows us to replicate the DNA, Multiple cloning site
You're Reading a Preview
Unlock to view full version