I clicker: normally, body fluids of fish in temperate waters have a freezing point somewhere between -0. 6 and -1. 1 c whereas seawater freezes at -1. 9 c. A. due to the noncolligative properties of the body fluid. Colligative: antifreezers lower freezing point of body fluids by increasing concentration of solutes. (ex. glycerol, propaline glycol) Noncolligative antifreezes lower the freezing point of body fluids because of specialized chemical properties. What strategies do arc ectotherms use to survive. Strategies freeze tolerance freeze intolerance: behavioural avoidance. X: production of antifreeze x x compounds, synthesis of ice x nucleating agents, supercooling (temperate. Hypothesis: in cold water marine fish, the trigger for synthesis of antifreeze protein is temperature. Prediction: if so, then if we lower the external temp and sample blood should be an increase in antifreeze protein. (decreasing water temp will increase [afp]). Experimental group: 20 fish, decrease temp to -1. 51 degrees(c) Observation: antifreeze proteins increase in fall and decrease in spring in arctic marine fishes.