BIOL 1090 Lecture Notes - Lecture 10: Prenylation, Ribosome, Dna Replication

• School: University of Guelph
• Department: Biology
• Course: BIOL 1090
• Professor: Andrew Bendall

Document Summary

Nucleus: characteristics, 5-10% of cell volume, ca. 10 um diameter: typically one/cell, functions of the nucleus, storage, replication and repair of genetic material, expression of genetic material, ribosome biosynthesis. Structure of the nucleus: nuclear envelope, nuclear membrane, nuclear lamina, nuclear pores, nuclear content, chromatin, nucleoplasm, nuclear matrix, nucleolus. Functions of nuclear envelope: separates nuclear content from cytoplasm, separates transcription and translation, selective barrier, allows limited movement of molecules between nucleus and cytoplasm, binds nuclear lamina. **mutations in lamin gene (lmna) implicated in hutchinson-gliford progeria syndrome (causes premature aging in children) Nuclear pores: nuclear function is dependent upon protein import. Nuclear pore complex (npc: protein structure fills nuclear pore, supramolecular complex, 100sof polypeptides, approximately 30 different nups, fits into the pore; reduces functional diameter to approximately 9 nm, extends into cytoplasm and nucleoplasm, octagonal symmetry. Gfp (27kd) are able to diffuse freely in and out.