BIOL 1090 Lecture Notes - Lecture 14: Aequorin, Cholesterol, Hydrophile

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Cell diagrams on textbooks = cells that are dead! A certain amount of energy is absorbed, then very quickly released at a slightly higher wavelength. The energy released will always be at a higher wavelength! Good to know when deciding what colour filter to use to see a certain colour fluorescence. Jellyfish appear transparent in nature! (b/c they match the wavelength of their surroundings! Soo when they get hit with light --> excites them and that"s why they will appear fluorescent: 1962 --> shimomura extracted aequorin & gfp (green fluorescent protein, gfp received nobel prize in 2008. Since then, maaaany creatures have been labeled with gfp! It exists in 4d! (x, y, z, t) --> b/c always changing! (so time component too!) The living cell is veery dynamic! (always in motion!) Eukaryotic cells have compartments (membrane-bound) and components (not membrane- bound) Organelle = a membrane-bound compartment with characteristic content and specific function.

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