BIOL 1090 Lecture Notes - Lecture 11: Caat Box, Transfer Rna, Stem-Loop
21 Aug 2016
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Document Summary
The mature mrna contains both coding and non-coding sequences! Many differences in the initiation/elongation/termination/processing of transcripts between. Tata box & caat box, -80 & -120 base pairs. Rna hairpin: followed by 6 a"s, polymerase falls off. Transcription factors first bind dna, then recruit polymerase. 5" cap: poly-a tail, mrna is further processed! Mrna is cleaved downstream of aauaaaa, near a g-u rich sequence. Mrna = the intermediate between dna & protein. The nature of the gene & its expression part 2 translation. 2 subunits! (ex: 30s* & 50s subunit) Most of ribosome = made of folded up rrna (ribosomal rna)! Ribosome is positioned at the 5" end. Begins translation at the beginning of coding sequence/open reading frame. Shine-dalgarno sequence (on mrna) pairs with the region of base-pairing (on ribosome) Cap-binding protein binds to the 5" cap, helps position the ribosome at the 5" end. Ribosome then scans progressively until finds aug and suitable surrounding sequence.
